Terraria Wiki → How to place Truffles in a biome
What we need to make a site and a house:
1. 135 blocks of dirt
2. A couple of seeds of glowing mushrooms
3. A place for construction (I made it at a height so as not to interfere)
4. 100-150 glowing mushrooms for building a house Truffle
Construction
1. We build a "floor" from dirt blocks 3 blocks high, 45 blocks long (As in the screenshot)
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2. We plant seeds of a glowing mushroom anywhere, in the center, in the corners, anywhere xD
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3. Wait 3-5 days for the seeds to germinate.
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4. From the mushrooms that we prepared earlier, we make a kind of "box", a table, a chair, a workbench, a door, walls, torches - that's all we need
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5. We wait for Truffle to move into our "box"
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Source: steamcommunity.com
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